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5.6 PHYTOTRON FACILITY Assessing the leaf area index and biomass production in mustard varieties by the use of thermal time

Based on the field experiments with two Brassica varieties (Pusa Jaikisan and Bio-169-96), thermal response curves were developed using thermal time as a base. The best-fit polynomial second order regression equations were developed taking LAI as a dependable variable with the thermal time, viz., growing degree days (GDD) as an independent variable. The growing degree days could explain 96% to 99% variation in LAI in different treatments in Pusa Jaikisan and Bio-169-96 in October 15 sowings and 95% to 98% in October 30 sowing. When the 2006-07 season data were pooled with the previous crop season data (two varieties and two seasons), the correlation coefficients were highly significant (at 1 per cent). Hence, the regression equations were developed which will be of immense use in assessing the leaf area index by the use of the thermal time, without destroying the plant samples. Similarly, by the use of thermal response curves of biomass production, regression equations were developed to assess the biomass production at different intervals of time, without destroying the plants. However, caution is required when the data over varieties are pooled, as they might lead to considerable errors. It would be worthwhile to develop equations, which are variety and location specific. These types of quantitative relationships would go a long way in dynamic crop simulation modeling studies.

Thermal response curves: (top) for leaf area index (LAI), and (bottom) for biomass in mustard under control(D0), and debranched at 50 DAS(D1) and at 60 DAS(D2)

Effect of debranching on ground shading inhibiting the radiation penetration to the ground level (left: control, right:debranched plots)

16. Functional genomics for chickpea – for drought screening (NRC on Plant Biotechnology)

17. Evaluation of transgenic cucumber lines against CMV-CP virus (Division of Plant Pathology)

18. Agroinoculation of tomato leaf curl virus clones on N.

benthaminana and tomato (Centre for Virology)

19. Transgenic tomato for post harvest (NRC on Plant Biotechnology)

20. Molecular characterization of Indian wheat germplasms for quality (Division of Genetics)

21. Transgenic cotton (cocker 310 F12) with Cry 1Ac gene for resistance to H. armigera (Delhi University, South Campus) 22. Growing T1 plants of transgenic brinjal (var. PPL)with Bt gene

for insect resistance (NRC on Plant Biotechnology) 23. Whole genome characterization of Indian Citrus ring spot virus

(Division of Plant Pathology)

24. Growing of break fern for phytoremediation of contaminated soil (Division of Soil Science & Agricultural Chemistry) 25. Functional genomics of chickpea – NFBSRA Project (NRC on

Plant Biotechnology)

26. Effect of Arbuscular Mycorrhiza Fungi and water stress in citrus (Division of Fruits & Horticultural Technology)

27. Screening of wheat genotypes for phosphorus use efficiency (Division of Plant Physiology)

28. Biofortification of wheat land races by molecular and conventional plant breeding methods (Division of Genetics) 29. Evaluation of PGPR-Cyanobacteria for wheat and paddy crops

(Division of Microbiology)

30. Marker assisted selection for seedling and adult plant leaf rust resistance in wheat (National Phytotron Facility)

31. Study on heat stress in Chenopodium album (Water Technology Centre)

32. Inheritance and mapping of disease resistance genes in lentil (Division of Genetics)

33. Use of molecular markers in wheat quality breeding (CCS University, Meerut)

34. Study of drought resistance in wheat (WL 711 x C 306) RILs (Water Technology Centre)

35. Grown T0 plant of Bt transgenic tomato cv. Pusa Ruby (NRC on Plant Biotechnology)

36. Development of transgenic maize for stem borer resistance (Directorate of Maize Research)

37. Physiological and biochemical bases of high temperature tolerance studies in chickpea genotypes (Division of Plant Physiology)

38. Transferred blast resistant transgenic rice plants from the net- house to Phytotron for molecular and functional analyses (NRC on Plant Biotechnology)

39. Salt stress study on rice (NRC on Plant Biotechnology) 40. Pathogenic and molecular variability of Bipolaris maydis

incitant of maydis leaf blight of maize (Division of Plant Pathology)

41. Rice crossing for F1 (NPT x PB 1) (NRC on Plant Biotechnology)

42. Tillering population (N 22) M2 (NRC on Plant Biotechnology) 43. Inoculation of bioformulation treated rice seedlings with xoo

(Division of Plant Pathology)

44. Development of pro-vitamin A rich indica rice through marker assisted backcross breeding (Division of Genetics)

45. T1 generation of tomato cultivar PED, having truncated A-rep gene for tomato leaf curl virus (Division of Plant Pathology) 46. Identification of promoter location and assessment of promoter

strength in Arabidopsis (NRC on Plant Biotechnology) 47. Effect of viral suppressors of RNAi on Arabidopsis (Centre

for Virology)

48. Functional analysis of carotenoid biosynthesis genes in tomato (NRC on Plant Biotechnology)

49. Development of transgenic papaya resistant to spot virus using coat protein gene (Advance Centre for Virology)

50. Gene pyramiding for multiple biotics stress resistance in crops (wheat) (Division of Genetics)

51. Physiological experiments in tomato NCPGR, JNU, New Delhi 52. Characterization and identification of bacterial genes imparting osmo-tolerance and their validation in plants (NRC on Plant Biotechnology)

53. Mapping of F2 populations and 21 lines – International Tomato Genome Sequencing Project (NRC on Plant Biotechnology) 54. Modeling epidemiology of F. monilifpormae var. subglutinans

(Division of Fruits & Horticultural Technology)

55. Microbial diversity of Rhizobium and isolation of genes for symbiosis and biotic resistance (NRC on Plant Biotechnology) 56. Evaluation of clodinafop, sulfosulfuron and pinoxaden towards cross resistance across Phalaris minor Retz. biotypes (Division of Agronomy)

57. Genetic transformation of wheat for drought tolerance (NRC on Plant Biotechnology)

58. Screening of transgenic rice plants agains Magnaportha grisea (NRC on Plant Biotechnology)

59. Isolation and characterization of PR genes in Brassica juncea against Alternaria brassicacea and study of their regulation (NRC on Plant Biotechnology)

60. Development of procedure and protocol for ascertaining of EDV status of wheat (Division of Genetics)

61. Transgenic Arabidopsis transplanted for tissue specific promoter validation (NRC on Plant Biotechnology) 62. Temperature dependent regulation of Fad2 gene experssion in

Brassica juncea (Division of Biochemistry)

63. Role of signal peptide in aphid resistance of Brassica (NRC on Plant Biotechnology)

64. Effect of silencing of nematode genes on infection on development and reproduction of Heterodera anenae - Silencing of nematode genes using RNAi (Division of Nematology)

65. Evaluation of the salt tolerance mechanism in Azolla sp.- Anabaena system (CCUBGAC)

66. Evaluation of fungicidal properties of Calothrix elukerii extracts against damping off disease caused by Pythium apharidermatum in vegetables (brinjal, chillies and tomato) (Division of Microbiology)

67. Proteome analysis of heat susceptible and tolerant wheat genotypes (Division of Genetics)

68. Molecular characterization of tn-5 mutants of Mesorhizobium ciceri (NRC on Plant Biotechnology)

69. Unravelling molecular processes involved in the adventive polyembryony towards genetic engineering for fixation of heterosis – Transfomation of Arabidopsis thaliana with promoter trap vector (NRC on Plant Biotechnology) 70. Senescence: Mechanism in crops in relation to abiotic stress-

sink strength and their interaction (Water Technology Centre)

71. Cloning and characterization of abiotic stress inducible TF gene in rice (NRC on Plant Biotechnology)

72. Effect of salicylic acid on high temperature stress amelioration on wheat during post flowering period (Division of Plant Physiology)

73. Genetic analysis of abiotic stress tolerance in Arabidopsis (Water Technology Centre)

74. Double haploid production in wheat (NRC on Plant Biotechnology)

75. Screening races against 40 A race of stem rust (Division of Genetics)

76. Treatment for synchronization of flowering in wheat genotypes (Division of Genetics)

77. Development of abiotic stress tolerant transgenic rice (NRC on Plant Biotechnology)

78. Testing the packaging material for the storage of Brocolli florets (Division of Post Harvest Technology)

79. Stress treatment at 6 oC to rice plants (NRC on Plant Biotechnology)

80. Expression analysis of transcription factors during abiotic stress in wheat (NRC on Plant Biotechnology)

81. Isolation and characterization of abiotic stress inducible transcription factor (promoters) in response to cold stress in rice (NRC on Plant Biotechnology)

82. Cloning and characterization of wound inducible promoter from Arabidopsis thaliana (NRC on Plant Biotechnology) 83. Development of mapping population for identification of QTLs

– Molecular breeding of PCR based marker for sheath blight resistance gene in rice (NRC on Plant Biotechnology) 84. Studies on the uptake of K, Co, Na from saline Hoagland

solution – Role of SOS pathway in salinity tolerance in wheat (Division of Plant Physiology)

85. Effect of Pseudomonas fluroscens on life cycle of Meloidogyne incognita (Division of Nematology)

86. Genetic transformation studies in pigeonpea with Bt genes (cv.

Pusa 855, tall) (NRC on Plant Biotechnology)

87. Molecular characterization of cryptic root specific promoter isolated from Arabidopsis thaliana (NRC on Plant Biotechnology)

88. Biochemical isolation of defense related signal peptides from rice – Isolation and characterization of defense peptides (Division of Agricultural Chemicals)

89. Development of transgenic tomatoes for resistance to fruit borer (NRC on Plant Biotechnology)

90. Molecular characterization of Indian wheat germplasm lines for quality traits – DNA isolation from leaves (Division of Genetics)

91. Studies on the efficiency of a biocontrol agent against Fusarium udhum in Cajanus cajan (Division of Microbiology) 92. Inheritance and mapping of disease resistance genes in lentil –

raise F1 generation (seed to seed) (Division of Genetics) 93. Multiplication of wild species of lens (Division of Genetics) 94. Studies on GBNV and TSV on groundnut – Maintenance of

GBNV and TSV on cowpea (Division of Plant Pathology)

95. Comparative molecular analysis of cmds lines of B. juncea carrying D. catholica cytoplasm – Validation through transgenic expression in Arabidopsis (NRC on Plant Biotechnology)

96. Molecular breeding in wheat improvement (Division of Genetics)

97. Differential expression profiling and EST development for leaf rust resistance (Division of Genetics)

98. Infectivity of Potato apical leaf curl virus on different hosts (Division of Plant Pathology)

99. Assessment of genetic vigour between poor and better storers of soybean lines (Division of Seed Science & Technology)

Development of transgenic Arabidopsis through A. tumefaciens using floral dip method: (a) Seeds of Arabidopsis after 10 days of inoculation on ½ MS, (b) plants of Arabidopsis after 30 days of seeds inoculation, (c) plants inoculated with A. tumefaciens, and (d) fully matured siliques covered with butter paper

Independent lines of transgenic cotton at the Phytotron facility

(C) (D)

(A) (B)