• No results found

2.3 Optical trapping in Ray-optics regime

2.3.2 Mathematical formulation of force calculation model in the ray optics

2.3. Optical trapping in Ray-optics regime


Optical axis



1aser b eam


Fig. 2.4 Schematic diagram representing ray-pencil model.

2.3.2 Mathematical formulation of force calculation model in the ray op-

Chapter 2: Force Calculation Model of Optical trapping


𝑃 𝑃′′




Optical axis

Spherical wavefront



Fig. 2.5 Schematic diagram depicting the spherical wavefront model.

where,zris the Rayleigh length and is given by zr= π ω02

λ (2.6)

Hereω0 is the beam waist and λ is wavelength of light. zp is the distance between S and P which is the z coordinate ofPand S is the position of beam waist. The model uses the approximation|SP|=|SP′′|, which is the case whenrP is very large or when the wavefront is almost planar. Net force on the particle is obtained by integrating over all the rays those are incident on the particle within the cone. The limit of the integration are obtained from the direction of⃗rp . However, the SWF model provides correct optical trapping force only under paraxial focusing conditions. On the contrary the ray-pencil model is applicable for both paraxial and strong focus conditions.

Force due to a single ray

Figure2.6(i) shows a single ray with powerdPincident on a dielectric sphere at an angle of incidenceα. The incident momentum isnmeddP/cwherenmed is the refractive index of the medium. Part of the incident ray gets reflected with powerdP Rand the refracted ray undergoes infinite number of reflections and refractions in succession with decreasing powers,dP T2, dP T2R,...., dP T2Rn. HereRand T are the Fresnel reflection and transmission coefficients,

2.3. Optical trapping in Ray-optics regime


𝛽 𝑜



𝑑𝑃𝑇2 𝑑𝑃𝑇2𝑅

𝜙 𝜓

𝜓 + 𝜙

Incident ray




𝐹𝑡𝑜𝑡 𝑦෡ 𝑧෡


𝑜 Ԧ𝑟

𝑏 Ԧ 𝑔

𝑥 𝑧





Fig. 2.6 Geometry for force calculation in the ray optics regime due to a single incident ray of powerdP. (i) shows the multiple reflections and refractions of the single ray incident on the spherical particle and (ii) shows the geometry to calculate the incident angle when the sphere is shifted by⃗rfrom the focus.

respectively. Due to the momentum change experienced by this single ray a force acts through the center of the sphere. This force can be divided into two componentsdFz acting along ˆz anddFy acting along ˆygiven as [38]

dFz =nmeddP

c −hnmeddP R

c cos(π−2α) +


nmeddP T2Rn

c cos(ψ+nφ)i



dFy=0−hnmeddP R

c sin(π−2α)−


nmeddP T2Rn

c sin(ψ+nφ) i

(2.8) We combine the two components in complex plane asdFtot =dFz +idFy, so that we can write

dFtot = nmeddP

c (1+Rcos 2α)−inmeddP R

c sin 2α−nmeddP T2 c


Rne−i(ψ+nφ) (2.9)

Chapter 2: Force Calculation Model of Optical trapping Taking real and imaginary parts we get

dFz= nmeddP c


1+Rcos(2α)−T2[cos(2α−2β) +Rcos(2α)]

1+R2+2Rcos(2β) i

(2.10) and

dFy =−nmeddP c


Rsin(2α)−T2[sin(2α−2β) +Rsin(2α)]

1+R2+2Rcos(2β) i

(2.11) More details about the derivation of the above force components can be found in Appendix A.

The forcedFz as given by Eq.2.10is called scattering force which acts in the propagation direction of the incident ray and the forcedFyas given by Eq.2.11is called the gradient force which acts perpendicular to the scattering force. Both the force components depend on the powerdPcarried by the ray, refractive index of the medium, angle of incidence, and angle of refraction. To be noted that since the Fresnel transmission and reflection coefficientsRand T depend on polarization of light [1] the two forces are also polarization dependent. If we consider the light to be randomly polarized or circularly polarized so that each ray has equal amount of s and p polarizations then we can write

R(α,β) =1 2

hsin(α−β) sin(α+β)


+1 2

htan(α−β) tan(α+β)


(2.12) and

T(α,β) =1−R(α,β) (2.13)

The net force on the sphere due to a single ray therefore is

d⃗F= (nmed/c)[Re(Qtrap)zˆ+Im(Qtrap)yˆ]dP (2.14) where,


1+Rexp(2iβ) (2.15)

is called as trapping efficiency. Hence, the force experienced by the dielectric sphere for a given ray can be calculated using Eq.2.14provided the respective(α,β)and(R,T)values are known. In this work we assumeRandT values as given by Eqs.2.12and2.13.

2.3. Optical trapping in Ray-optics regime

Net force due to a tightly focused Gaussian beam

Considering all the rays incident on the sphere the net force on it can be obtained by vectorially addingd⃗F due to each ray. Therefore,


dF (2.16)


nmedc [Re(Qtrap)zˆ+Im(Qtrap)yˆ]dP (2.17)

The elementary power elementdPdepends on the intensity distribution at the entrance pupil of the objective lens. In this thesis we denote the components of the net force alongx,yandzas Fx,FyandFz.

In most of the cases the intensity distribution at the entrance pupil is considered as Gaussian distribution in which case the intensity at the locationρ(x,y)in the pupil plane is given as

I(x,y) =Ioexp(−ρ2

2) (2.18)

whereσ is the beam waist andρ=p

x2+y2. We are expressing the power using cylindrical co- ordinates as the Gaussian distribution has a cylindrical symmetry. The power in the elemental area dAat (x,y) in the pupil plane isdP = I(ρ)dA, which is also taken as the power in the corresponding ray passing through(x,y). IfPtot is the net power in the Gaussian beam then

dP= Ptot

2π σ2exp(− ρ22)dA

= Ptot

2π σ2exp(− ρ2

2)ρdρdϕ (2.19)

since in cylindrical polar coordinates, the elementary areadA=ρdρdϕ. Now, considering the objective lens obeys Abbe’s sine condition, that is


x2+y2= fsinθ (2.20)

Chapter 2: Force Calculation Model of Optical trapping

where,θ is the angle the ray makes with the optical axis of objective lens. Thus the angle made by the ray with thezaxis is decided by the radial distance from the axis in the pupil plane.


dP= Ptot

2π σ2exp(−f2sin2θ

2 )f2sinθ cosθdθdϕ (2.21) Substituting Eq.2.21in Eq.2.17we get [8]








c [Re(Qtrap)zˆ+Im(Qtrap)yˆ] Ptot

2π σ2exp(−f2sin2θ

2 )f2sinθ cosθdθdϕ (2.22) In the above Eq.2.22the unit vectors are expressed as

= (−sinθcosϕ,−sinθsinϕ,−cosθ) (2.23)


= zˆ×(⃗r×zˆ)

|zˆ×(⃗r×zˆ)| (2.24) The expression of the incident angleα can be derived using Fig.2.6(ii) as

α =cos−1hg2+b2−r2 2bg




g= q

b2−r2+r2(sinχsinθcosϕ+cosχcosθ)2 +r(sinχsinθcosϕ+cosχcosθ)


Using Snell’s law

β =sin−1 nmed

ns sinα

(2.27) where,nsis the refractive index of sphere. The value ofθ0is decided by the numerical aperture of the focusing lens or the critical angle of incidence on the glass coverslip in case the specimen slide containing the dielectric sphere uses a glass coverslip, which ever is smaller. Thus the

2.4. Augmented ray-pencil model to calculate optical forces