f M '•• C M F R I SPECIAL P U B L I C A T I O N
N u m b e r 7MANUAL OF RESEARCH MiiTHODS FOR CRUSTACEAN BIOCHEMISTRY AND PHYSIOLOGY
ISSIH;(! o n Hie o c c a s i o n of the W o t k s h o p ott C R U S T A C E A N B I O O H f c M I S T H Y A N D P H Y S I O L O G Y
j o i n t l y ocyriiHseil by
t h e D e p a r t m e n t <sf Z o o l o g y , Oniv<-r,iiy of Madras . m i l the Contra of A d v a n c e d S t u d i e s so M a r i o u l t t m - ,
C e n t r a l Marine f i s h e r i e s li.-s^at,. h i u s h f t t t c , held at Madras. f r o m 8 •• 20 J tin- 1 **tt1
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MANUAL OF RESfARI CRUSTACEAN BIOCHEMI!
THODS FOR D PHYSIOLOGY
Workshop on IIOCHEMISTRY A N D PHYSIOLOGY
•eparSrient o f $ o 3 # j i y J k t v e r s i t y of Madras and Cefliiji of Adv«ri<f»d«udies in Mariculture,
intraf llarfne Fish#fMWRese,jrch Institute, held at tAadtg*Ji&tn\- 20 June 1981
Manual dfRp^rch Methods for
!( ^Crustacea* ^chmmtt^md Physiology ,
EDITED BY
Mr H. RAVINDRANATH
*cAtw/ <r/ tmkabbhgy, Department ofZoabgy, University of Madras, Maims 600 005
CMFRI SPECIAL PUBLICATION
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ISSUED ON TOT OCCASIDH OJP TUB WORKSHOP ON BIOCHEMISTRY AlfO ^fBJJflQM^^MlMlMfc.
DEPARTMENT 0*"gj£ioi]M' ""•
CENTRE OP ADVAJfeWv^taK^r'tw''
MARINE FISHERIES RESeAROH INSTITUTE U M t ^
',7' *"'*1&%
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(LIMITED DISTRIBUTION)
Published by : E. G. SILAS
Director
Central Marine Fisheries
Research Institute I Cochin 682018 I
PRINTED IN INDIA i : AT THE DIOCESAN PRESS, MADRAS 6 0 0 0 0 7 — 1 9 8 1 . C 2 3 7 5 .
.s; T'-rzjv^:^. ^^b»YWEIGHt,'"WATERAND ASH CONTENTS *
2.1 PRINCIPLE
The wet (fresh) tissue is allowed to dry by keeping it in a desic- cator. The difference between the wet weight of the tissue and its dry weight gives the amount of water present in the fresh -tksu4P(Paft»oneaii & Williams;1 1953 ; Mullainadhan, 1979).
On1 heating the dry material to higher temperature (550-600°C) all the organic constituents are burnt leaving only the inorganic , constitueats in the form of ash.
2.2 PROCEDURE
1. Take a small amount of tissue on previously weighed plastic slip.
2. Find out the weight of the tissue (=wet weight).
3. Keep the tissue along with the plastic slip in a desiccator maintaining 0-5% R.H.
4. Dry the tissue in the desiccator till it reaches a constant weight.
5. Keep the dry material in a porecelain crucible and heat it at 550-600°C in an incinerator for 4 hoars.
6. Find out the weight of the ash.
2.3. CALCULATIONS
2.3.1. Dry weight Dry weight
Wet weight x 100 = /100 gm wet weight (gm %)
* Prepared and verified by P. Mullainadhan, School of Pathobiology, Department of Zoology, University of Madras, Madras-600 005.
2.3.2. Water content
2.3.3. Ash content
ff Sgf * 10 ° " /I0 ° gm dry wei * ht <** %)
2.4 REFERENCES
MULLAINADHAN, P. 1979. Haemolymph water, volume-aad tissue water in Scylla serrata Forskal (Crustacea : Decapoda). M. Phil.
Dissertation, University of Madras, p. 67.
PASSONEAU, J. V. & C. M. WILLIAMS, 1953. The molting fluid of the Cecropia silkworm. / . Exp. Biol., 3 0 : 545-560.
8
F^ryour
l..|l.1^II.IM—III I — . . . . own notes.
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