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KINETIC STUDIES OF CODTINUOUS FERMENTAION OF SACCHARIFIED CELLULOSE TO ETHYL ALCOHOL

BY

RAJESHWAR DAYAL TYAGI

THESIS SUBMITTED TO THE INDIAN INSTITUTE OF TECHNOLOGY,DELHI FOR THE AWARD OF THE DEGREE OF

DOCTOR OF PHILOSOPHY

BIOCHEMICAL ENGINEERING RESEARCH CENTRE CHEMICAL ENGINEERING DEPARTMENT INDIAN INSTITUTE OF TECHNOLOGY,DELHI

NEW DELHI-110029 NOVEMBER 1978

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CERTIFICATE

This is to certify that the thesis entitlec "Kinetic Studies of Continuous Fermentation of Saccharified Cellulose to Ethyl Alcohol" by Shri Rajeshwar.Dayal Tyagi has been prepared under my supervision in conformity with the rules and regulations of the

Indian Institute of Technology, Delhi, I further certify that the thesis has attained a standard requirec for Ph.D. Degree of the Institute. The research report one results presented in the thesis have not been submitted for any degree in any other University.

72,

(T .K oGhos e

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ACKNOWLEDGEMENT

I express my sincere gratitude to my research supervisor, Prof. Tarun K. Ghose, for his expert guidance, stimulating

discussion, continued encouragement and keen interest throughout the period of this investigation.

I am grateful to Drs. S.K.Tangnu and P.Ghosh, M/S Vikram Sahai, Subhash Chanel, P.S,Vishnoi, Deepak Jain,

R.K.Sachdev, and other friends and colleagues for their valuable suggestions and enthusiastic cooperation,

M/S S.Sen Ray anc V.K,Ghosh are to be than- Ked for their skillful technical help. Appreciation is also

extenc ec to Mr. G.N.Sharma for his willing assistance.

Finally, Mr. R.N,Shukla is thanked who patiently typed this manuscript.

0,7#114 (R.D.TYAGI)

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ACKNOWLEDGEMENT

express my sincere gratitude to my research supervisor, Prof. Tarun K. Ghose, for his expert guidance, stimulating

discussion, continued encouragement and keen interest throughout the perioc of this investigation.

I am grateful to Drs. S.K.Tangnu and P.Ghosh, M/S Vikram Sahai, Subhash Chand, P.S.Vishnoi, Deepak Jain,

R.K.Sachdev, and other friends and colleagues for their valuable suggestions and enthusiastic cooperation,

M/S S.Sen Ray and V.K.Ghosh are to be thankec for their skillful technical help. Appreciation is also

extenc ec to Mr. G.N.Sharma for his willing assistance.

Finally, Mr. R.N,Shukla is thanked who patiently typec this manuscript,

65?

' TbIa#

(R .D .TYAGI )

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CONTENTS

CHAPTER - I Page No.

SECTION - I

1,1 INTRODUCTION ... 1 1.2 OBJECTIVE ...14 SECTION - II

1.3 LITERATURE SURVEY ,1

7

CHAPTER - 2

SECTION - I

2,1 MATERIALS AND ASSAY

METHODS ...29 SECTION - II

2.2 EXPERIMENTAL ...40 SECTION - III

2.3 PRODUCTION OF BAGASSE

HYDROLYSATE ...48 CHAPTER - 3

SECTION -

3.1 KINETIC STUDIES IN BATCH AND CONTINUOUS

CULTURE . . .54

SECTION - II

3.2 RAPID FERMENTATION OF BAGASSE HYDROLYSATE SECTION - III

3.3 EFFECTS OF OXYGEN ON

ETHANOL PRODUCTIVITY ...89

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CHAPTER - 4

SECTION 4 . SECTION

4 . 1

2 - I

- II

PRODUCT I \II-11BIT= ,

SUBSTRATE INHIBITION

94

. ..100 CHAPTER - 5

SECTION - I

5 . 1 PREDICTION OF BATCH

DATA FROM THE PROPOSED

!MODEL . ..104

SECTION - II

5 . 2 OPT I= DESIGN OF

FERMENTOR . . . 1 10

SECTI 0 \I - III

5 . 3 GRAPHICAL ANALYSIS ...120 CHAPTER — 6

SECTION - 1

6 . 1 CONCLUSIONS . .125

SECTION - II

6 . 2 S UNDATRY . ..131

APPENDICES ( 1 to 4) NO E \ICLATURE

REFERENCES

References

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